For best-in-class results, use best-in-class tools

Twist Bioscience is a leading and rapidly growing biotechnology company that has developed a disruptive DNA synthesis platform to industrialize the engineering of biology. The core of the platform is a proprietary technology that pioneers a new method of manufacturing synthetic DNA by “writing” DNA on a silicon chip. Twist is leveraging its unique technology to manufacture a broad range of synthetic DNA-based products, including synthetic genes, tools for next-generation sequencing (NGS) preparation, and antibody libraries for drug discovery and development.

Meet Twist Bioscience

Twist’s Precision Dx Products

Introducing Twist’s Precision Dx Products — a whole exome library preparation and target enrichment workflow with IVDR certification.

Whole exome sequencing (WES) has become crucial in clinical practice, enabling a comprehensive approach to identifying genomic variants that may contribute to a diagnosis or guide treatment decisions. With greater efficiency than whole genome sequencing and broad coverage over protein-coding sequences, WES can be an ideal tool for genomic analyses.

This suite of products includes:

●    Twist Precision Prep and Enrichment Dx Kit: 
Reagents for enzymatic fragmentation, library preparation, and target enrichment.

●    Twist Precision Exome Dx Kit:

Reagents for enzymatic fragmentation, library preparation, and target enrichment along with targeted capture probes for exome enrichment.

●    Twist Precision Exome Dx Panel:

Targeted capture probes for exome enrichment.

The Twist Precision Prep and Enrichment Dx Kit and the Twist Precision Exome Dx Panel products can be purchased individually or they can be ordered as a complete solution, which is the Twist Precision Exome Dx Kit.

The Twist Precision Exome Dx Products are intended for use with high-quality genomic DNA derived from human cells such as whole blood, ultimately producing exome-enriched libraries compatible with Next-Generation Sequencing (NGS) applications. The Twist Precision Dx Product offers an EU IVDR-compliant solution with proven performance. 

IVDR Compliant

Demonstrated quality of kit materials
▪ Compliant with EU IVDR 2017/746 regulations

Reliable performance

Consistent yield from high-complexity libraries
▪ Uniform enrichment of target regions
▪ Sequence verified probes produced

Comprehensive Coverage

Covers 37.7Mb of the human genome
 >97% coverage of the CCDS, RefSeq, Gencode and Ensembl v101 database
▪ Expanded coverage of mitochondrial genome

Twist Exome 2.0

Twist Exome 2.0 is designed to detect rare and inherited diseases, as well as germline cancers. This panel’s high uniformity and low off-target rate deliver best-in-class sequencing efficiency, enabling quality data to be collected with less sequencing. With superior coverage of major genetic databases (RefSeq, CCDS, GenCode, Clinvar, ACMG73 and more) and the addition of clinically relevant non-coding pathogenic and likely pathogenic variants, Twist Exome 2.0 provides the value of multiple clinical panels all wrapped into one, easily customizable package.

Coverage That Matters

Covers 36.5 Mb of human protein coding regions
Based on recent database releases
Includes carefully curated clinical content

Best-in-Class Performance

Uniform enrichment of target regions
NGS QC of all probes

Retain Flexibility

Easily spike-in content into the exome
Effective across multiplex target enrichment workflows
Run overnight or same day workflows

Twist cfDNA Library Preparation Kit

High Sensitivity. High Conversion. Low-Input

The Twist cfDNA Library Preparation Kit addresses challenges associated with library preparation from circulating cell-free DNA (cfDNA). Liquid biopsies have gained prominence in oncology research, particularly for investigating potential biomarkers within genetic material from tumors circulating in peripheral blood. This kit enables high conversion cfDNA library generation for next-generation sequencing (NGS) on Illumina systems, overcoming the technical challenges associated with low-input and degradation of cfDNA, as well as other samples that are hard to acquire and have limited DNA abundance (i.e. urine and CSF samples).

The Twist cfDNA Library Preparation Kits provide our highest quality library preparation solution, offering not only robust performance with low sample input, but also high conversion rates.

Maximize Rare Variant Detection

▪ High conversion rate reliably detects rare variants (≤0.1% VAF)
▪ High coverage libraries compatible with duplex sequencing workflows supported by the Twist UMI Adapter System

Streamline Workflow of Precious Samples

▪ Robust performance, even with low (<1ng) sample input
▪ Sequence-ready libraries in 2 hours

Twist Custom Panels

Company Twist Bioscience combines precise oligonucleotide synthesis with a scalable silicon-based manufacturing platform to generate high-performing probe panels for NGS target enrichment.

They also complement this powerful technology with proprietary design algorithms and a rapid iteration pipeline to enable the quick design, synthesis, and optimization of custom panels.

Twist Custom Panels can be designed and built to cover a wide range of panel sizes, target regions, and multiplexing requirements — all with the exceptional and consistent performance you’ve come to expect from Twist NGS solutions.

With their incredibly flexible design capabilities, you can easily expand or enhance content on your existing panel or blend together the content from multiple panels with the Twist Custom Blended Panels option. This unprecedented flexibility saves you time while guaranteeing high-quality performance and analysis.

Whether you design your own panel from scratch or add targets to enhance the content of existing panels, you can use your Twist Custom Panel with Twist’s modular library preparation kits or seamlessly integrate them into an existing workflow.

Rapid Customization and Optimization

▪ Fast turnaround time to final panel
▪ Simple addition or enhancement of content without sacrificing performance
▪ Ensures adjustment of workflow biases for optimum performance

Exceptional, Consistent Performance

▪ Effective design, accurate synthesis, and detailed quality control maximize both uniformity of capture and reproducibility
▪ High-fidelity double-stranded DNA probes offer high-quality, consistent performance
▪ NGS-based quality control of all probes ensures balanced probe representation and minimizes dropout

Intuitive, Proprietary Panel Design

▪ Scalable design from 100 to >1M probes per pool
▪ Reduces the iterations to final design
▪ Enables balanced capture, even for challenging regions

And a Lot More

Achieve superior enrichment efficiency with Twist Bioscience NGS Target Enrichment Solutions. Targeted sequencing takes a step forward with an optimized enrichment workflow and easy customization of panel content. Twist offers a wide range of NGS solutions, from library preparation from DNA and RNA, fixed panels, methylation detection and a lot more. Check out their website or contact us for more information.

Contact – Responsible Manager

Kristýna Slabá

Business Development Manager – Oncology

E-mail: slaba@3genes.com
Mobile: +420720358655

Tomáš Straka

Business Development Manager – Diagnostics, IVF

E-mail: straka@3genes.com
Mobile: +420733202307

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