High data volume
Up to 2,500 human genomes per year at 20× coverage and up to 480 Gb of HiFi data per run. Four independent SMRT Cells with flexible run scheduling.
PacBio flagship
Revio: long‑read sequencing at scale
Revio is a high-throughput long-read sequencer with HiFi technology and 99.95% (Q33) sequencing accuracy. Up to four SMRT Cells in parallel, 2,500 human genomes per year and methylation detection directly from native DNA, with no bisulfite conversion and no systematic errors.
- 2,500 Human genomes per year
- 4 SMRT Cells running in parallel
- Q33 High HiFi accuracy (99.95%)
- 480 Gb HiFi data per run
Key advantages of Revio
More HiFi data. Lower costs.
HiFi quality in every read
99.95% accuracy (Q33), 90% of bases above Q30 and uniform coverage regardless of GC content. A single experiment gives you all variant types: SNVs, indels, structural variants and repeat expansions.
Methylation with no extra work
Every standard run detects 5mC, 5hmC and 6mA methylation directly from native DNA. No bisulfite, no separate library, no extra-cost chemistry.
Easy operation
Starting a run takes less than a minute, and library preparation is ready for automation. Because the work deck is separate from the four sequencing positions, the instrument can be operated at practically any time while it is running.
20× more computing power
Onboard NVIDIA GPUs and the DeepConsensus algorithm from Google Health. Twenty times more computing power than the Sequel IIe. You receive HiFi data in BAM format for downstream analysis.
The quality of HiFi technology
HiFi — long‑read accuracy on par with short reads
Circular consensus sequencing (CCS) reads the same native DNA molecule repeatedly and builds a single, highly accurate long read from it. No PCR, no systematic errors.
Read length up to 25 kb
Covers entire genes, regulatory regions and structural variants in a single read.
Read accuracy
Thanks to repeated reading, accuracy reaches 99.95% (Q33).
Reliable phasing
Phased haplotypes directly from long reads: complete information without additional analyses.
De novo assembly
De novo assembly with quality comparable to reference genomes, including repetitive regions.
Handles complex regions
Resolves highly homologous regions, segmental duplications and centromeres, where short reads fail.
Native methylation in every run
Detection of 5mC, 5hmC and 6mA directly from native DNA. No bisulfite conversion needed.
Technical specifications
Revio specifications
Sequencing (SPRQ-Nx)
- Read length
- 0.5–25 kb (depending on library)
- Read accuracy
- 99.95% (Q33)
- Bases above Q30
- 90–95% (depending on library length)
- HiFi yield / SMRT Cell
- 100–120 Gb
- Run time
- 12–30 hours
- Methylation detection
- 5mC, 5hmC and 6mA from native DNA
Throughput
- Genomes per year (12 h runs)
- 2,500 WGS
- Genomes per year (24 h runs)
- 1,250 WGS
- HiFi data per run
- up to 480 Gb
- Parallel SMRT Cells
- 4 independent positions
Instrument
- Dimensions (W × D × H)
- 92.7 × 91.4 × 174.5 cm
- Weight
- 465 kg
- Power supply
- 200–240 V AC, 50/60 Hz
- Maximum power draw
- 5 kVA / 4.8 kW / 24 A
- Operating temperature
- 19–25 °C
- Humidity
- 20–80% RH, non-condensing
Compute & data
- Onboard computing power
- NVIDIA GPUs (20× vs Sequel IIe)
- Network
- 1 GbE / 10 GbE copper
- Output format
- HiFi reads in BAM format
- Data per SMRT Cell
- ~60 GB
- Annual data volume
- up to 78 TB
Suitable applications
HiFi performance for a wide range of applications
Human genetics
Two human genomes per SMRT Cell at 20× coverage. Simultaneous detection of SNVs, indels and structural variants.
Rare diseases
Higher diagnostic yield in paediatrics and neonatology. Detection of repeat expansions and structural variants in a single run.
Oncology
Somatic SNVs, indels and structural variants, clonal phasing in tumours and MRD detection.
Microbiome and metagenomics
16 metagenomic samples per SMRT Cell. Up to 384 microbial genomes per SMRT Cell at 30× coverage.
Iso-Seq (Kinnex)
Up to 6 samples per SMRT Cell and up to 10 million reads. Analysis of complete isoforms and full-length single-cell RNA.
Epigenetics and Fiber-seq
8 samples per SMRT Cell at 5× coverage. Mapping of chromatin architecture and detection of 5mC, 5hmC and 6mA in every run.
Q2/2026
SPRQ-Nx — the new sequencing chemistry!
SPRQ-Nx is a new, improved generation of HiFi sequencing chemistry. Its key innovation is multi-use SMRT Cells, which are reusable sequencing cells. Lower consumable use, a lower cost per genome and improved methylation detection.
With the new SPRQ-Nx chemistry, SMRT Cells can be reused, reducing sequencing costs by up to 30%!
- ↑ Output
- More HiFi reads from each SMRT Cell
- ↑ 5hmC
- Hydroxymethylation detection
- Multi-use
- Reusable SMRT Cells
- Q33
- HiFi accuracy maintained
Reagents and library preparation
Reagents compatible with Revio
Nanobind DNA Extraction Kits
Extraction of native DNA from saliva, cultured cells, blood, tissue, plant nuclei, insects and bacteria.
SMRTbell prep kit 3.0
Universal library preparation for WGS, targeted sequencing and other applications. Manual or automated.
Kinnex full-length RNA kit
Iso-Seq for full-length mRNA transcript analysis and single-cell transcriptomics.
PureTarget panels
Targeted HiFi panels for comprehensive analysis of difficult-to-sequence regions.
Ampli-Fi
Amplicon sequencing with DNA input from as little as 1 ng. Over 1,000 samples per SMRT Cell.
Revio sequencing reagents
SMRT Cells, a sequencing plate and polymerase: everything you need for HiFi sequencing.
Watch
Revio in action
Join us as we start a sequencing run.
Let's get started
Would you like Revio in your laboratory?
We will prepare a no-obligation consultation, a demo and an operating-cost calculation tailored to your projects. We provide installation, training, and application and service support.