HiFi technology on your bench
The first benchtop long-read sequencer with 99.95% HiFi accuracy. The same data quality as Revio, in a format that fits into an ordinary laboratory.
Benchtop HiFi platform
Vega: accessible HiFi sequencing
Vega is the first benchtop sequencer with PacBio HiFi technology. The same long reads and 99.95% accuracy as Revio, in a compact format. Perfect for smaller projects and accessible to smaller laboratories too.
- 300 Human genomes per year
- 1 SMRT Cell
- Q33 High HiFi accuracy (99.95%)
- 90 Gb HiFi data per run
Key advantages of Vega
Easier access to HiFi technology
No waiting
One SMRT Cell per run, with no waiting for a pool to fill. The next run can be set up in advance for continuous operation.
On-instrument analysis
Base calling, HiFi read generation, methylation detection and export to BAM all take place directly on the instrument.
Plug-and-play installation
Standard 230 V power supply and 1 GbE network. Intuitive workflow with minimal risk of error.
Compact sequencing data
Efficient storage of sequence data, quality values and methylation at approximately 0.5 bytes per base, up to 20× more compact than other long-read technologies. Output of approximately 30 GB of data per run.
The quality of HiFi technology
HiFi — long‑read accuracy on par with short reads
Circular consensus sequencing (CCS) reads the same native DNA molecule repeatedly and builds a single, highly accurate long read from it. No PCR, no systematic errors.
Read length up to 25 kb
Libraries from 0.5 to 25 kb. They cover entire genes, regulatory regions and structural variants in a single read.
Read accuracy
Thanks to repeated reading, accuracy reaches 99.95% (Q33).
Reliable phasing
Phased haplotypes directly from long reads: complete information without additional analyses.
De novo assembly
De novo assembly with quality comparable to reference genomes, including repetitive regions.
Handles complex regions
Resolves highly homologous regions, segmental duplications and centromeres, where short reads fail.
Native methylation in every run
Detection of 5mC, 5hmC and 6mA directly from native DNA. No bisulfite conversion needed.
Technical specifications
Vega — specifications
Sequencing (SPRQ-Nx)
- Read length
- 0.5–25 kb (depending on library)
- Read accuracy
- 99.95% (Q33)
- Bases above Q30
- 90–95% (depending on library length)
- Methylation detection
- 5mC, 5hmC and 6mA from native DNA
- Run time
- 2–24 hours
- DeepConsensus
- On the instrument
Throughput
- Output per run
- Up to 90 Gb
- Capacity / year
- ~200 SMRT Cells
- Human genomes / year
- Up to 300
- Microbial samples per run
- ~384
Instrument
- Dimensions (W × D × H)
- 55.7 × 69.5 × 76.8 cm
- Weight
- 125.2 kg
- Power supply
- 200–240 V AC, 50/60 Hz
- Operating temperature
- 19–25 °C
- Humidity
- 20–80% RH, non-condensing
Compute & data
- On-instrument analysis
- Base calling, HiFi, methylation, BAM
- Network
- 1 GbE
- Output format
- HiFi reads in BAM format
- Data representation
- ~0.5 byte / base (20× more compact)
- Software
- SMRT Link (web-based)
Suitable applications
What you can sequence on Vega
Human genetics
WGS with the full spectrum of variants: SNVs, indels, structural variants and repeat expansions. Up to 300 genomes per year at 20× coverage.
Rare diseases
Higher diagnostic yield in paediatrics and neonatology. Detection of repeat expansions and structural variants in a single run.
Microbiome and metagenomics
Up to 384 microbial genomes per SMRT Cell at 30× coverage. Metagenomics and complete bacterial genomes, including plasmids.
Iso-Seq (Kinnex)
Enables full-length transcript analysis and single-cell transcriptomics with full isoform resolution.
Targeted panels
PureTarget panels for clinical research and oncology. Targeted gene panels with high coverage.
Epigenetics and methylation
5mC in CpG context and 6mA from native DNA in every standard run. Mapping of chromatin architecture.
Q3/2026
SPRQ-Nx — the new sequencing chemistry!
Vega uses the new, improved generation of SPRQ chemistry, which increases the output from a single SMRT Cell from 60 to 90 Gb.
Library preparation and workflow are fully shared by both PacBio sequencers. What works on Revio also works on Vega: compatible reagents, kits and analysis pipeline.
- ↑ Output
- More HiFi reads from each SMRT Cell
- ↑ 5hmC
- Hydroxymethylation detection
- ↓ Cost
- Lower cost per genome
- Q33
- HiFi accuracy maintained
Reagents and library preparation
Reagents compatible with Vega
Nanobind DNA Extraction Kits
Extraction of native DNA from saliva, cultured cells, blood, tissue, plant nuclei, insects and bacteria.
SMRTbell prep kit 3.0
Universal library preparation for WGS, targeted sequencing and other applications. Manual or automated.
Kinnex full-length RNA kit
Iso-Seq for full-length mRNA transcript analysis and single-cell transcriptomics.
PureTarget panels
Targeted HiFi panels for comprehensive analysis of difficult-to-sequence regions.
Ampli-Fi
Amplicon sequencing with DNA input from as little as 1 ng. Over 1,000 samples per SMRT Cell.
Vega sequencing reagents
SMRT Cells, a sequencing plate and polymerase: everything you need for HiFi sequencing.
Watch
Vega in action
Get to know the new Vega sequencer.
Let's get started
Would you like Vega in your laboratory?
We will prepare a no-obligation consultation, a demo and an operating-cost calculation tailored to your projects. We provide installation, training, and application and service support.