Higher yield
Higher library yield from low-input and degraded FFPE samples. Higher ligation conversion efficiency and fewer samples that need reanalysis.
Twist Bioscience
Twist TrueAmp LP Kit — optimised for challenging samples
A universal library prep kit from Twist Bioscience with the unique TrueAmp polymerase. Higher yield from low-input and FFPE samples, uniform coverage across GC extremes and higher library complexity than competing kits.
- 1 ng Ultra-low input gDNA
- FFPE Compatible with degraded DNA
- ↑ Yield Higher yield than competitors
- ↑ Complex Higher library complexity, fewer PCR duplicates
What is the TrueAmp LP Kit
Library prep kit with the unique TrueAmp polymerase
The Twist TrueAmp Library Preparation Kit is a universal NGS library preparation kit designed for subsequent target enrichment. Its key innovation is TrueAmp polymerase, a high-fidelity enzyme developed for uniform amplification even in challenging regions.
The kit handles inputs from 1 ng up to 500 ng. It is compatible with fresh gDNA, FFPE tissue (even with DIN < 2.2), cfDNA and mixed inputs. Consistent results across samples without the need for per-sample optimisation.
Key advantages
Five features that make TrueAmp stand out
Uniform GC coverage
TrueAmp polymerase maintains consistent coverage in both high-GC and low-GC regions, even with a higher number of PCR cycles.
Higher library complexity
Preserves more unique DNA molecules for sequencing. Fewer PCR duplicates and higher variant detection sensitivity.
FFPE compatibility
Optimised for degraded samples with DIN < 2.2. Consistent fragment length across samples without the need for optimisation.
Universal workflow
One kit for target enrichment, hybrid capture, WGS, WES, liquid biopsy and MRD. Compatible with the UMI adapter system.
How it works
Three-step workflow
01
Enzymatic fragmentation
Extracted DNA is precisely fragmented with a selectable insert size (fragmentation time and temperature can be adjusted for different sample types).
02
Adapter ligation
An optimised ligase maximises conversion efficiency and minimises ligation bias. Compatible with both the universal and the UMI adapter system.
03
TrueAmp amplification
High-fidelity amplification with TrueAmp polymerase increases both yield and variant detection sensitivity, even with a higher number of PCR cycles.
Comparison
Measured values compared with competitors
Charts comparing TrueAmp with a competing polymerase on FFPE samples with low DNA input.
Yield, library size and target coverage
Twist TrueAmp achieves a significantly higher DNA concentration (4× more ng/μl), comparable library size and higher mean target coverage than competitor polymerase K on FFPE samples with low DNA input.
Coverage uniformity and zero-coverage targets
TrueAmp provides a higher percentage of bases at the required coverage depth and reduces the number of target regions with zero coverage.
Coverage across GC content
Normalised coverage at 3 and 16 PCR cycles. TrueAmp maintains consistent coverage in both GC-rich and GC-poor regions, where competing products drop off.
Library size across input amounts
Consistent fragment size from ultra-low (1 ng) to standard (500 ng) inputs. Electropherograms demonstrate reproducibility.
Selectable fragment size
The insert size can be selected by changing the fragmentation time and temperature, giving flexibility for different downstream applications.
Watch
TrueAmp in action
A short video introduces the Twist TrueAmp Library Prep Kit workflow: three steps from extracted DNA to a finished library ready for sequencing.
Let's start
Would you like TrueAmp in your lab?
Send us a few details about your workflow (sample type, application, volume) and we will be happy to discuss everything with you and prepare a cost estimate. We can also offer you a trial at a discounted price.