Detection at ≤ 0.1% VAF
High-conversion library preparation and low-bias TrueAmp amplification enable sensitive detection of rare variants in cfDNA, ideal for early cancer detection and MRD.
Twist Bioscience
Twist cfDNA TrueAmp Kit — library ready in 2 hours
The Twist cfDNA Library Preparation Kit with its unique TrueAmp polymerase is a dedicated kit for library preparation from cfDNA. It is ready for liquid biopsy, MRD monitoring and pan-cancer screening, with variant detection at ≤ 0.1% VAF and error rates on a par with PCR-free libraries.
- < 1 ng cfDNA input
- 2 h Total time to a finished library
- ≤ 0.1% VAF detection limit for rare variants
- UMI Fully compatible
About the product
Library preparation tailored to cell-free DNA
The Twist cfDNA Library Preparation Kit with TrueAmp polymerase is optimised for fragmented, low-input cfDNA. The workflow skips the fragmentation step and uses an engineered T4 DNA ligase with high conversion efficiency together with TrueAmp polymerase for amplification with minimal bias.
The kit achieves error rates on a par with PCR-free libraries, despite the amplification step. This enables detection of low-frequency variants at ≤ 0.1% VAF. Fully compatible with Twist UMI adapters.
- Engineered T4 DNA ligase for high conversion efficiency
- TrueAmp polymerase: error rates on a par with PCR-free libraries
- Consistent fragment size from 37.5 ng to 300 ng input
- Balanced GC content: minimises bias at GC extremes
Key advantages
Five properties that make the cfDNA TrueAmp Kit stand out
Quality and speed
The substitution error rate matches PCR-free workflows, with the speed of a 2-hour kit and extremely low DNA input.
Higher library yield
Consistently higher yield than competing kits, even at 15 ng input and with 0.25% VAF standards.
Duplex sequencing with UMIs
Full compatibility with the Twist UMI Adapter System for duplex sequencing: highly sensitive detection in complex applications.
Hybrid-capture ready
Compatible with Twist target enrichment panels: Oncology DNA CGP, MRD Express and custom panels. Standard hybridisation protocol.
Workflow
From cfDNA to sequencing analysis
The Twist cfDNA Library Prep Kit covers both the library prep and hybrid capture steps. The library is ready in 2 hours.
01
Sample prep
Isolation of cfDNA from plasma. No further fragmentation required.
02
Library prep
Engineered T4 ligase + Twist Universal/UMI adapters. TrueAmp PCR (typically 6 cycles).
03
Target enrichment
Hybrid capture with any off-the-shelf or custom panel.
04
Sequencing
Illumina-compatible libraries for short-read sequencing.
05
Analysis
Variant calling with UMI deduplication for detection at ≤ 0.1% VAF.
Measured values
Comparison with competing products
Charts comparing the Twist cfDNA TrueAmp Kit with competing kits K, Q, W and N on 15 ng input standards.
Error rate on a par with PCR-free libraries
The substitution error rate of Twist TrueAmp amplification matches PCR-free methods, without compromising workflow speed.
Detection sensitivity at 0.25% VAF
Higher sensitivity and accuracy of variant detection at 0.25% VAF across different substitution types, which is critical for liquid biopsy.
Library complexity with UMIs
Higher library complexity (with and without UMI deduplication) preserves more unique molecules for reliable duplex sequencing analysis.
Main applications
What you can do with cfDNA TrueAmp
Liquid biopsy
cfDNA analysis for early cancer detection: detection of ctDNA at low VAF (≤ 0.1%), even in complex clinical samples.
MRD monitoring
Monitoring of residual disease in plasma. Compatible with the Twist MRD Express panel for real-time relapse detection.
Pan-cancer screening
Testing for a broad spectrum of solid tumours from a single blood tube. Compatible with the Twist cfDNA Pan-Cancer Reference Standard v2.
Duplex sequencing
Highly sensitive detection with UMI duplex sequencing, which combines both DNA strands and sharply reduces the error rate.
Oncology CGP
Comprehensive genomic profiling: SNVs, indels, CNVs and biomarkers from the same library.
NIPT and fetal DNA
A compatible workflow for analysing fetal cfDNA from maternal blood, suitable for research NIPT studies.
Let's start
Would you like to try the cfDNA TrueAmp Kit?
Tell us about your application and we will be happy to discuss the options with you.