Kits tailored to the application
Each kit is tuned to a specific use: microbial genomes, agriculture, single-cell methods, WGS. No one-size-fits-all compromises.
Simple workflow · Scalable sequencing
seqWell — plate-based NGS library preparation for large numbers of samples
seqWell is a US manufacturer of plate-based NGS library preparation kits. The kits are built on its proprietary TnX™ transposase, which allows a library to be prepared in a single day with sample normalisation built into the protocol. The kits run in both 96- and 384-well formats, from tens to thousands of samples a month.
- 384 Wells per plate, scaling from 96
- 1 day Complete library prep
- 85% On-target reads after optimisation
- 95–99% Prep success rate
About the company
NGS libraries prepared on a plate
seqWell builds kits for laboratories that prepare thousands of sequencing libraries a year. The aim is to eliminate routine pipetting and the normalisation of each sample individually. Every kit is optimised for a specific input type, number of samples and downstream application.
The platform handles short and long reads, low and standard inputs, and both columns and deep-well plates. Two-day protocols are thus shortened to a single day. QC and normalisation steps move to the level of the whole plate, so a technician can process 96 samples as easily as eight.
Technology
TnX™ transposase and a plate-based workflow
TnX™ is a new-generation tagmentation transposase. Compared with standard Tn5 enzymes, it delivers more uniform coverage, higher yield and greater library complexity. Combined with a plate-based protocol, it removes the need to normalise individual samples: samples are pooled in balanced proportions directly from the plate.
One-day library prep
A traditional two-day protocol is shortened to a single day. Faster time to data and fewer shifts per run.
Built-in normalisation
Normalisation takes place directly on the plate. There is no need to measure every sample on a Qubit or TapeStation before pooling.
From 96 to 384 wells
The same protocol runs on both 96-well and 384-well plates. Capacity increases fourfold with no change to the SOP and no retraining.
More uniform coverage
TnX™ delivers more uniform coverage than standard Tn5 enzymes. Fewer dropouts and cleaner downstream analysis.
Fewer operator errors
Fewer standalone pipetting steps and fewer intermediate QC steps. Preparation success rate of 95–99%.
Applications
Where seqWell delivers libraries
Low-pass WGS
Whole-genome screening runs with tens to thousands of samples: population studies, imputation, ancestry determination.
Microbial genomics
Bacterial whole-genome sequencing, strain tracking and pathogen surveillance. High sample numbers at a low cost.
Agrigenomics
Panels for plants and livestock: GBS and WGS for breeding programmes. AgriPrep was developed specifically for this area.
Plasmids and amplicons
Plasmid verification, amplicon sequencing and genome editing QC. Fast QC runs without lengthy protocols.
SARS-CoV-2 surveillance
Pathogen surveillance with proven repeatability. Protocols used in epidemiological studies during the pandemic.
Single-cell methods and antibodies
High-volume library preparation for single-cell methods and antibody development.
Portfolio
Key products from seqWell
ExpressPlex™ 2.0
LongPlex™
MosaiX™
AgriPrep™
Tagify™ (Custom / i5 UMI / TnX Universal)
PhiRx™
Let's get started
Interested in seqWell for your lab?
We will help you choose a kit based on your application, number of samples and sequencer. Consultations are non-binding and available in the Czech Republic and Slovakia. We can also offer evaluation samples.