Official PacBio representative in the Czech Republic, Slovakia, Poland and Hungary
PacBio (Pacific Biosciences) is a US manufacturer of sequencing platforms focused on high-accuracy long-read technology. Its HiFi sequencing combines the best of the short-read and long-read worlds: long reads, high accuracy and a comprehensive view of the genome.
PacBio offers two platforms built on the same HiFi chemistry, for laboratories and projects of different sizes.
Flagship · Population scale
A high-throughput long-read system for large projects. Up to 2,500 human genomes per year on a single instrument, bringing long-read sequencing to the scale of population studies.
Compact · Benchtop sequencer
A benchtop long-read sequencer that brings the same HiFi accuracy to smaller laboratories. A benchtop format, a simple workflow and a lower initial investment, with no compromise on data quality.
PacBio builds technologies for scientists and clinicians who need a complete view of the genome, from individual bases through structural variants to methylation.
Whole-genome and targeted panel sequencing for clinical research, rare-disease diagnostics and population studies.
Plant and animal research using accurate long-read sequencing delivers actionable genomic insights for addressing global challenges.
Uncover the complex composition of microbial communities and gain deeper insight into metagenomics with accurate long-read sequencing.
Enables comprehensive analysis of tumour genomes, epigenomes and transcriptomes with a single technology.
In each run, PacBio circularises the DNA and reads the same fragment repeatedly. The resulting consensus eliminates random errors and produces highly accurate long-read data.
HiFi sequencing takes place on unique SMRT Cells (Single Molecule, Real-Time), in wells called ZMWs (Zero-Mode Waveguides). The polymerase reads the native DNA molecule in real time: no PCR, no amplification bias.
The SMRTbell library joins the ends of the molecule into a circle. The polymerase then travels around this circular DNA repeatedly, so a single molecule yields 8–15 independent reads.
A consensus is calculated from the repeated passes: a single, highly accurate long read with a Q33+ score. Methylation information from the native DNA is preserved at the same time.
A single read spans entire genes, regulatory regions and structural variants that are beyond the reach of short-read technologies.
Circular consensus sequencing reads the same molecule repeatedly, resulting in short-read-level accuracy.
Epigenetic information directly from native DNA, with no need for chemical conversion or a separate library.
SNVs, indels, structural variants, repetitive regions and phasing, all from a single experiment.
Accurate de novo assembly of genomes without a reference sequence.
Consistent results across instruments and runs, which is critical for clinical and population applications.
New in 2026
SPRQ-Nx is the latest generation of PacBio sequencing chemistry, taking HiFi technology even further. It delivers a higher data yield from each SMRT Cell and better accuracy, while remaining fully compatible with existing workflows.
With the new SPRQ-Nx chemistry, SMRT Cells can be reused, reducing sequencing costs by up to 30%.
How HiFi technology is changing the way laboratories around the world sequence.
We will prepare a no-obligation consultation, a demo and an operating-cost calculation tailored to your projects. We provide installation, training, and application and service support.
